A first step in calibrating a whole-slide fluorescence cytometer is using a uranyl slide proving spatial uniformity to spatially normalize LED illumination and camera vignetting. We then measure the coefficient of variation (CV) for each pixel, expecting the CV to increase away from the center of slide as in the following Blue and UV excited fluorescence images.
In this data, the estimated CV is over N</var≥50 images and 1.3 ms exposure. The plots are created by PlotCV.py.
The next step is to test the system using fluorescent beads of known diameter, where the CV will be worse as a figure of merit vs. other systems.